Through PPI and hub gene selection, we obtained 7 additional robust hub genes, CCND2, WT1, E2F2, IRF1, BAZ2A, LAMC1, and DAB2.
Results: We successfully identified 282 DElncRNAs and 380 DEmRNAs through differential analysis, and we constructed an OSA-related ceRNA network consisting of 292 miRNA-lncRNAs and 41 miRNA-mRNAs. Then, we conducted LASSO regression analysis on the stable hub genes, selected relatively significant genes to construct a simple and easy-to-use nomogram, validated the nomogram, and constructed the core ceRNA sub-network of key genes.
We then constructed lncRNA-related ceRNA networks, performed functional enrichment analysis and protein-protein interaction analysis, and performed internal and external validation of the expression levels of stable hub genes. Based on the differential expression of lncRNA, we identified the target genes of miRNA that bind to lncRNAs. Methods: We collected plasma samples from OSA patients and healthy controls for the detection of ceRNA using a chip. We intend to identify the potential hub genes for the development of OSA, which will provide a foundation for the study of the molecular mechanism underlying OSA and for the diagnosis and treatment of OSA. Objectives: Some ceRNA associated with lncRNA have been considered as possible diagnostic and therapeutic biomarkers for obstructive sleep apnea (OSA). 2Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.1The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.Wang Liu 1 †, Xishi Sun 2 †, Jiewen Huang 1, Jinjian Zhang 1, Zhengshi Liang 1, Jinru Zhu 1, Tao Chen 1, Yu Zeng 1, Min Peng 1, Xiongbin Li 1, Lijuan Zeng 1, Wei Lei 2* and Junfen Cheng 1*
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